Tn(CAG-cas9*-SPH)1Smoc
Transgene Detail

Summary

• Symbol: Tn(CAG-cas9*-SPH)1Smoc
• Name: transposon insertion 1, Shanghai Model Organisms Center
• MGI ID: MGI:7292766
• Transgene: Tn(CAG-cas9*-SPH)1Smoc Location: unknown
• Alliance: Tn(CAG-cas9*-SPH)1Smoc page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Transposon induced (Endonuclease)
• Mutation: Transposon insertion
• Mutation details: Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher). (J:238766)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:238766 Shanghai Model Organisms Center, Information obtained from the Shanghai Model Organisms Center (SMOC), Shanghai, China. MGI Direct Data Submission. 2017
• All: 1 reference(s)