Gt(ROSA)26Sor^{em52(CAG-KRAB,-cas9*,-EGFP)Smoc}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em52(CAG-KRAB,-cas9*,-EGFP)Smoc}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 52, Shanghai Model Organisms Center
• MGI ID: MGI:7290061
• Synonyms: Gt(ROSA)26Sor^{em1(CAG-LSL-KRAB-dCas9-IRES-EGFP)Smoc}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em52(CAG-KRAB,-cas9*,-EGFP)Smoc} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Endonuclease, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: These mice harbor a CAG-LSL-3xKRAB-dCas9-IRES-EGFP-WPRE-pA conditional expression cassette in mouse Rosa26 loci via homologous recombination. This strain can be used in CRISPR mediated target gene transcriptional inhibition (CRISPRi). The loxp-stop-loxp cassette was deleted. (J:238766)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:238766 Shanghai Model Organisms Center, Information obtained from the Shanghai Model Organisms Center (SMOC), Shanghai, China. MGI Direct Data Submission. 2017
• All: 1 reference(s)