Tfrc^{em1(TFRC)Dnli}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tfrc^{em1(TFRC)Dnli}
• Name: transferrin receptor; endonuclease-mediated mutation 1, Denali Therapeutics
• MGI ID: MGI:7286478
• Synonyms: TfR^mu/hu
• Gene: Tfrc Location: Chr16:32427738-32451612 bp, + strand Genetic Position: Chr16, 23.06 cM
• Alliance: Tfrc^{em1(TFRC)Dnli} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tfrc^{em1(TFRC)Dnli} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TFRC (7037)

  Tfrc	(MGI:98822)

  The transferrin receptor apical domain is human, rest is mouse.

• Mutation details: CRISPR/Cas9 technology knocked in the human transferrin receptor apical domain coding sequence into the locus creating a chimeric mouse/human transferring receptor under the control of the endogenous promotor which preserves the native murine transferrin ligand binding domain and retains the majority of Tfrc introns, minimizing potential dysregulation of protein expression and function. (J:325423)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tfrc Mutation: 61 strains or lines available

References

• Original: J:325423 Kariolis MS, et al., Brain delivery of therapeutic proteins using an Fc fragment blood-brain barrier transport vehicle in mice and monkeys. Sci Transl Med. 2020 May 27;12(545)
• All: 4 reference(s)