Bard1^tm8.1Rjbr
Targeted Allele Detail

Summary

• Symbol: Bard1^tm8.1Rjbr
• Name: BRCA1 associated RING domain 1; targeted mutation 8.1, Richard J Baer
• MGI ID: MGI:7284161
• Synonyms: Bard1-AAE
• Gene: Bard1 Location: Chr1:71066690-71142300 bp, - strand Genetic Position: Chr1, 35.67 cM
• Alliance: Bard1^tm8.1Rjbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: KV1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: The targeting vector contains a loxP-flanked neomycin inserted into intron 3 and the following AAE amino acid substitutions - phenylalanine to alanine at position 125 (F125A, TTT to GCT), aspartic acid to alanine at position 127 (D127A, GAT to GCT), and alanine to glutamic acid at position 128 (A128E, GCA to GAA) - inserted into exon 4. The mutations correspond to human mutations (F133, D135, F136) that disrupt the interaction of BARD1 with Rad51 and impair the strand-invasion step of homologous recombination. Cre-mediated recombination removed the neo cassette. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bard1 Mutation: 54 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)