Tcf4^em3Bdph
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tcf4^em3Bdph
• Name: transcription factor 4; endonuclease-mediated mutation 3, Benjamin Philpot
• MGI ID: MGI:7280774
• Synonyms: Tcf4^LGSL, Tcf4^STOP
• Gene: Tcf4 Location: Chr18:69476500-69821038 bp, + strand Genetic Position: Chr18, 43.23 cM, cytoband E2
• Alliance: Tcf4^em3Bdph page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A cassette, comprised of a LoxP site, adenovirus splice acceptor, porcine teschovirus-1 2A (P2A) site, EGFP coding sequence, 3 copies of SV40 polyadenylation sequence (Stop), FRT site, and another LoxP site was inserted into intron 17. The sequence of the guide RNA (gRNA) was 5- GTCGTGCCTTACGTAGCTGGG- 3. (J:308040)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcf4 Mutation: 55 strains or lines available

References

• Original: J:308040 Kim H, et al., Region and Cell Type Distribution of TCF4 in the Postnatal Mouse Brain. Front Neuroanat. 2020;14:42
• All: 2 reference(s)