Nckap1l^tm1Iri
Targeted Allele Detail

Summary

• Symbol: Nckap1l^tm1Iri
• Name: NCK associated protein 1 like; targeted mutation 1, Brian M Iritani
• MGI ID: MGI:7279293
• Gene: Nckap1l Location: Chr15:103362221-103407237 bp, + strand Genetic Position: Chr15, 58.96 cM
• Alliance: Nckap1l^tm1Iri page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314432
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector PG00089_Y_3_F01 from KOMP was electroporated into ES cells. The construct is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 12-16 at position 103382899. The critical exons 12-16 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:314432)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nckap1l Mutation: 53 strains or lines available

References

• Original: J:314432 Suwankitwat N, et al., The actin-regulatory protein Hem-1 is essential for alveolar macrophage development. J Exp Med. 2021 Apr 5;218(4)
• All: 1 reference(s)