Hnmt^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hnmt^{tm1c(KOMP)Wtsi}
• Name: histamine N-methyltransferase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7279265
• Synonyms: HNMT^f
• Gene: Hnmt Location: Chr2:23892922-23939406 bp, - strand Genetic Position: Chr2, 16.06 cM
• Alliance: Hnmt^{tm1c(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0109_4_D09
• Germline Transmission: Earliest citation of germline transmission: J:324455
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 23935417 of Chromosome 2 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 23936162. The critical exon 3 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene and generated a "conditional read" allele with exon 3 floxed. (J:324455)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hnmt Mutation: 19 strains or lines available

References

• Original: J:324455 Otsuka R, et al., Contribution of astrocytic histamine N-methyltransferase to histamine clearance and brain function in mice. Neuropharmacology. 2022 Jul 1;212:109065
• All: 1 reference(s)