Atg16l1^tm1.1Umr
Targeted Allele Detail

Summary

• Symbol: Atg16l1^tm1.1Umr
• Name: autophagy related 16 like 1; targeted mutation 1.1, Ulrike Mayer
• MGI ID: MGI:7279133
• Synonyms: Atg16L1^deltaWD, atg16l1^E230
• Gene: Atg16l1 Location: Chr1:87683730-87720150 bp, + strand Genetic Position: Chr1, 44.44 cM
• Alliance: Atg16l1^tm1.1Umr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:284788
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two stop codons in exon 6 were inserted in frame at the end of the sequences encoding the coiled-coil domian (CCD). This mutation allowed translation of glutamate E226 and E230 residues in the CCD required for WIPI2 binding and autophagy, while the linker region and WD (tryptophan-aspartic acid) domain are not expressed. An frt-flanked neomycin selection cassette was removed via FLP-mediated recombination in the germline. (J:284788)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atg16l1 Mutation: 44 strains or lines available

References

• Original: J:284788 Rai S, et al., The ATG5-binding and coiled coil domains of ATG16L1 maintain autophagy and tissue homeostasis in mice independently of the WD domain required for LC3-associated phagocytosis. Autophagy. 2019 Apr;15(4):599-612
• All: 7 reference(s)