Gt(ROSA)26Sor^{em3(CAG-Nelfb)Bcgen}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em3(CAG-Nelfb)Bcgen}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 3, Biocytogen LLC
• MGI ID: MGI:7278804
• Synonyms: Gt(ROSA)26Sor^{em1(CAG-Nelfb)Bcgen}, Nelfb^Tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em3(CAG-Nelfb)Bcgen} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em3(CAG-Nelfb)Bcgen} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nelfb (MGI:1931035)

• Mutation details: The gRNA to Gt(ROSA)26Sor, a donor vector containing CAG promoter-loxP-Stop-loxP-mouse Nelfb cDNA-polyA, and Cas9 mRNA were co-injected into fertilized mouse eggs to generate targeted knockin offspring. (J:324288)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:324288 Wu B, et al., RNA polymerase II pausing factor NELF in CD8(+) T cells promotes antitumor immunity. Nat Commun. 2022 Apr 20;13(1):2155
• All: 1 reference(s)