C3ar1^tm1.1Hgr
Targeted Allele Detail

Summary

• Symbol: C3ar1^tm1.1Hgr
• Name: complement component 3a receptor 1; targeted mutation 1.1, Peter Heeger
• MGI ID: MGI:7277812
• Synonyms: C3ar1^fl
• Gene: C3ar1 Location: Chr6:122824099-122833116 bp, - strand Genetic Position: Chr6, 57.93 cM, cytoband F1
• Alliance: C3ar1^tm1.1Hgr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314460
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 122826325 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 122828577. The critical exon(s) is/are thus flanked by loxP sites. Flp-mediated recombination creates a "conditional ready" (floxed) allele. (J:314460)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C3ar1 Mutation: 34 strains or lines available

References

• Original: J:314460 Cumpelik A, et al., Dynamic regulation of B cell complement signaling is integral to germinal center responses. Nat Immunol. 2021 Jun;22(6):757-768
• All: 3 reference(s)