Ins2^em1Kplr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ins2^em1Kplr
• Name: insulin II; endonuclease-mediated mutation 1, John Kappler
• MGI ID: MGI:7275270
• Synonyms: Ins2-proinsulin-R(B22)E
• Gene: Ins2 Location: Chr7:142232393-142233463 bp, - strand Genetic Position: Chr7, 88.0 cM
• Alliance: Ins2^em1Kplr page

Mutation origin

• Strain of Origin: NOD

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Guide RNA and a mutant ssDNA oligonucleotide template are designed to create a CGT to a GAG mutation, resulting in an arginine to glutamic acid substitution at position 46 (R46E) in the insulin B chain (B22) of the insulin II (Ins2) gene [R(B22)E]. A silent mutation was also added, creating an Alu I restriction site. (J:324187)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ins2 Mutation: 90 strains or lines available

References

• Original: J:324187 Alam M, et al., Predisposition to Proinsulin Misfolding as a Genetic Risk to Diet-Induced Diabetes. Diabetes. 2021 Nov;70(11):2580-2594
• All: 1 reference(s)