Idh1^em2Nbar
Endonuclease-mediated Allele Detail

Summary

• Symbol: Idh1^em2Nbar
• Name: isocitrate dehydrogenase 1 (NADP+), soluble; endonuclease-mediated mutation 2, Nabeel Bardeesy
• MGI ID: MGI:7263364
• Synonyms: Idh1^R132C
• Gene: Idh1 Location: Chr1:65197775-65225638 bp, - strand Genetic Position: Chr1, 32.91 cM
• Alliance: Idh1^em2Nbar page

Mutation origin

• Strain of Origin: 129S6

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Using CRISPR-Cas9 technology in Idh1^em1Nbar zygote, a floxed STOP cassette (Lox-STOP-Lox) into the mouse genomic Idh1 locus upstream of a modified exon 3 engineered to contain a nucleotide substitution in codon 132 to cause the conversion of LSL-Idh1R132H allele to the LSL-Idh1R132C. Four sequence- and (R)-2HGvalidated founder lines were selected for further experimental study. This is a representative line used for the study. (J:321567)

Tumor Data

List all tumor models in MMHCdb carrying Idh1^em2Nbar

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Idh1 Mutation: 41 strains or lines available

References

• Original: J:321567 Wu MJ, et al., Mutant IDH Inhibits IFNgamma-TET2 Signaling to Promote Immunoevasion and Tumor Maintenance in Cholangiocarcinoma. Cancer Discov. 2022 Mar 1;12(3):812-835
• All: 1 reference(s)