Rag1^em1Dgs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rag1^em1Dgs
• Name: recombination activating 1; endonuclease-mediated mutation 1, David G Schatz
• MGI ID: MGI:7262701
• Synonyms: R1delta215
• Gene: Rag1 Location: Chr2:101468627-101479846 bp, - strand Genetic Position: Chr2, 53.88 cM
• Alliance: Rag1^em1Dgs page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 mediated recombination targeting the far N-terminusencoding region of Rag1 to replace the sequence encoding the initial 215 aa of RAG1, such that upon homology-directed repair-mediated genomic integration, the protein will begin at aa 216. (J:323218)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rag1 Mutation: 133 strains or lines available

References

• Original: J:323218 Beilinson HA, et al., The RAG1 N-terminal region regulates the efficiency and pathways of synapsis for V(D)J recombination. J Exp Med. 2021 Oct 4;218(10):e20210250
• All: 2 reference(s)