Slc12a5^tm2.1Dlp
Targeted Allele Detail

Summary

• Symbol: Slc12a5^tm2.1Dlp
• Name: solute carrier family 12, member 5; targeted mutation 2.1, Eric Delpire
• MGI ID: MGI:7261348
• Synonyms: Kcc2^lox
• Gene: Slc12a5 Location: Chr2:164802766-164841651 bp, + strand Genetic Position: Chr2, 85.28 cM, cytoband G2-G3
• Alliance: Slc12a5^tm2.1Dlp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292640
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site, a neomycin resistance gene cassette, and a second loxP site, were inserted 5' to exon 5, and a third loxP site was inserted 3' to exon 5. Exon 5 encodes the end of the second transmembrane domain (TM2) and a portion of the intracellular loop between TM2TM3. Deletion of this exon results in a preterm stop codon and complete KCC2 knockout. The neomycin cassette was removed via Cre-mediated recombination in the germline of mice, leaving loxP sites flanking exon 5. (J:292640)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc12a5 Mutation: 46 strains or lines available

References

• Original: J:292640 Mavrovic M, et al., Loss of non-canonical KCC2 functions promotes developmental apoptosis of cortical projection neurons. EMBO Rep. 2020 Apr 3;21(4):e48880
• All: 3 reference(s)