Casp8^tm2.1Gne
Targeted Allele Detail

Summary

• Symbol: Casp8^tm2.1Gne
• Name: caspase 8; targeted mutation 2.1, Genentech
• MGI ID: MGI:7260237
• Synonyms: Casp8^cC362A
• Gene: Casp8 Location: Chr1:58834553-58886663 bp, + strand Genetic Position: Chr1, 29.19 cM, cytoband B
• Alliance: Casp8^tm2.1Gne page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:285940
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site was inserted into intron 8 and an FRT site flanked neomycin resistance gene cassette and a second loxP site downstream of exon 10. A modified exon 9 where cysteine codon 362 (TGC) was mutated to alanine (p.C362A), together with a copy of the wild-type exon 10 and intervening intron 9, was inserted downstream of the gene and the 3' loxP site. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional allele with endogenous exons 9 and 10 floxed. When these exons are deleted through cre-mediated recombination, the allele will express the mutated p.C362A version of the gene. (J:285940)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casp8 Mutation: 45 strains or lines available

References

• Original: J:285940 Newton K, et al., Activity of caspase-8 determines plasticity between cell death pathways. Nature. 2019 Nov;575(7784):679-682
• All: 1 reference(s)