Pcolce^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pcolce^{tm1d(EUCOMM)Wtsi}
• Name: procollagen C-endopeptidase enhancer protein; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7259865
• Synonyms: PCPE1^-
• Gene: Pcolce Location: Chr5:137603369-137609666 bp, - strand Genetic Position: Chr5, 76.59 cM
• Alliance: Pcolce^{tm1d(EUCOMM)Wtsi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:322653
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 137604738 of Chromosome 5 upstream of the critical exons 4-7b (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 4-7b at position 137607834. The critical exons 4-7b are thus flanked by loxP sites. Flp-mediated recombination generated a "conditional ready" (floxed) allele. Subsequent cre expression deleted exons 4 to 7b resulting in a knock-out mouse. (J:322653)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcolce Mutation: 69 strains or lines available

References

• Original: J:322653 Sansilvestri-Morel P, et al., Deficiency of Procollagen C-Proteinase Enhancer 1 in Mice has No Major Impact on Cardiac Collagen and Function Under Basal Conditions. J Cardiovasc Pharmacol. 2021 Nov 1;78(5):e703-e713
• All: 2 reference(s)