Gt(ROSA)26Sorem1(CAG-Brainbow2.1,-Notch1)Koo
Endonuclease-mediated Allele Detail
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Symbol: |
Gt(ROSA)26Sorem1(CAG-Brainbow2.1,-Notch1)Koo |
Name: |
gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Bon-Kyoung Koo |
MGI ID: |
MGI:7259821 |
Synonyms: |
Red2-Notch1ICD |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sorem1(CAG-Brainbow2.1,-Notch1)Koo page
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Allele Type: |
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Endonuclease-mediated (Conditional ready, Inserted expressed sequence, Reporter) |
Mutation: |
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Insertion
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Mutation details: CRISPR-targeting inserted the Red2Onco construct, containing Notch1ICD (addgene 15079), into the tdimer2 reporter within the
Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle. The Brainbow2.1 (also known as Confetti) reporter construct is a modification of the Brainbow reporter with a strong CAGG promoter, a loxP site, a PGK-Neo-pA cassette (serving as a transcriptional roadblock), and coding region composed of two adjacent floxed head-to-tail tandem dimers. The first head-to-tail dimer contains a loxP site and humanized Renilla GFP (hrGFPII; with nuclear localization signal plus polyA sequence) in forward orientation and a loxP site and monomeric EYFP (mYFPA206K plus polyA sequence) in reverse orientation. The second head-to-tail dimer contains a loxP site and tdimer2 RFP plus polyA sequence in forward orientation, and a loxP site and mCerulean CFP (with membrane tethering palmitoylation sequence plus polyA sequence) in reverse orientation. A single frt site is located at the 3' end of the reporter construct.
(J:322623)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gt(ROSA)26Sor Mutation: |
945 strains or lines available
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Original: |
J:322623 Yum MK, et al., Tracing oncogene-driven remodelling of the intestinal stem cell niche. Nature. 2021;594(7863):442-447 |
All: |
3 reference(s) |
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