Parg^{tm2c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Parg^{tm2c(KOMP)Mbp}
• Name: poly (ADP-ribose) glycohydrolase; targeted mutation 2c, Mouse Biology Program, UC Davis
• MGI ID: MGI:7257820
• Gene: Parg Location: Chr14:31923906-32019507 bp, + strand Genetic Position: Chr14, 19.4 cM, cytoband B
• Alliance: Parg^{tm2c(KOMP)Mbp} page
• IMPC: Parg gene page

Mutation origin

• Mutant Cell Line: DEPD00583_7_A08
• Germline Transmission: Earliest citation of germline transmission: J:322048
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 31929646 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 31932826. FLP excised by microinjecting 100 ng/ul of FLP mRNA (Miltenyi BIotec Inc. Cat# 130-106-769) into the cytoplasm of IVF-derived zygotes. This resulted in the removal of the neo selection cassette and lacZ reporter leaving behind the floxed critical exon(s). (J:322048)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Parg Mutation: 68 strains or lines available

References

• Original: J:322048 The Centre for Phenogenomics, Direct Data Submission for The Centre for Phenogenomics Alleles. MGI Direct Data Submission. 2022
• All: 2 reference(s)