Gata1^tm1.1Itl
Targeted Allele Detail

Summary

• Symbol: Gata1^tm1.1Itl
• Name: GATA binding protein 1; targeted mutation 1.1, inGenious Targeting Laboratory
• MGI ID: MGI:7257809
• Synonyms: GATA-1^2RA, Gata1-KI
• Gene: Gata1 Location: ChrX:7825504-7842844 bp, - strand Genetic Position: ChrX, 3.59 cM, cytoband A2
• Alliance: Gata1^tm1.1Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:310648
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv x C57BL/6

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Arginine codons 243 (CGG) and 247 (CGA) in exon 4 were changed to alanine (GCA) (p.R243A, p.R247A) and an FRT site flanked neomycin resistance gene cassette was inserted into intron 4. The neo cassette was removed through subsequent flp-mediated recombination. The mutated arginine residues are essential for binding to the Hdac1 protein but their mutation doesn't affect other binding properties. (J:310648)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gata1 Mutation: 45 strains or lines available

Notes

HF4 (129S/SvEv x C57BL/6) ES cells

References

• Original: J:310648 Yan B, et al., HDAC1 is required for GATA-1 transcription activity, global chromatin occupancy and hematopoiesis. Nucleic Acids Res. 2021 Sep 27;49(17):9783-9798
• All: 1 reference(s)