Camk2a^tm2Yelg
Targeted Allele Detail

Summary

• Symbol: Camk2a^tm2Yelg
• Name: calcium/calmodulin-dependent protein kinase II alpha; targeted mutation 2, Ype Elgersma
• MGI ID: MGI:7254975
• Synonyms: Camk2a-LSL
• Gene: Camk2a Location: Chr18:61058704-61121224 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Camk2a^tm2Yelg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:321822
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A cassette containing Camk2a-exon2 fused to tdTomato, followed by the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), flanked by LoxP sites, and a neomycin cassette for positive selection, flanked by Frt sites was inserted upstream of Camk2a exon2. This results in a KO first mutant mouse line expressing tdTomato from the endogenous Camk2a promoter. Crossing this line with a cre line restores CAKM2A expression. (J:321822)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Camk2a Mutation: 136 strains or lines available

References

• Original: J:321822 Elgersma Y, Direct Data Submission for Camk2a-LSL. MGI Direct Data Submission. 2022
• All: 2 reference(s)