Gria1^tm8.1Rlh
Targeted Allele Detail

Summary

• Symbol: Gria1^tm8.1Rlh
• Name: glutamate receptor, ionotropic, AMPA1 (alpha 1); targeted mutation 8.1, Richard Huganir
• MGI ID: MGI:7254895
• Synonyms: SEP-GluA1 KI
• Gene: Gria1 Location: Chr11:56902342-57221070 bp, + strand Genetic Position: Chr11, 34.51 cM
• Alliance: Gria1^tm8.1Rlh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:321813
• Parent Cell Line: MC1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed to fuse a pH-sensitive super ecliptic pHluorin (SEP) variant of green fluorescent protein (GFP) sequence to the extracellular N-terminus by insertion into exon 1. A loxP-flanked neomycin resistance (neo) cassette was inserted downstream of exon 1. Cre-mediated recombination removed the loxP-flanked neo cassette. (J:321813)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gria1 Mutation: 66 strains or lines available

References

• Original: J:321813 Graves AR, et al., Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors. Elife. 2021 Oct 18;10:e66809
• All: 3 reference(s)