Nlrp3^em1Bfpy
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nlrp3^em1Bfpy
• Name: NLR family, pyrin domain containing 3; endonuclease-mediated mutation 1, Benedicte F Py
• MGI ID: MGI:7254890
• Synonyms: Nlrp3^S803D
• Gene: Nlrp3 Location: Chr11:59432395-59457781 bp, + strand Genetic Position: Chr11, 37.73 cM, cytoband B1.3
• Alliance: Nlrp3^em1Bfpy page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: Using a crRNA (UCUGAUUCCAAAGUCCCCCAGUUUUAGAGCUAUGCUGUUUUG) and an ssODN template (TGTCCCATCAATGCTGCTTCGACATCTCCTCTGTCCTGAGCAGCAGCCAGAAGCTGGTGGAGCTGGACCTCgaTGACAATGCaCTGGGGGACTTTGGAATtcGATTGCTGTGTGTGGGACTGAAGCACCTGCTCTGCA) with CRISPR/Cas9 technology, serine codon 803 (AGT) was changed to aspartic acid (GAT) (p.S803D). This mutation is phosphomimetic for residue 803. (J:313659)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nlrp3 Mutation: 69 strains or lines available

References

• Original: J:313659 Niu T, et al., NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly. Nat Commun. 2021 Oct 6;12(1):5862
• All: 1 reference(s)