Trp53bp2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Trp53bp2^{tm1c(EUCOMM)Hmgu}
• Name: transformation related protein 53 binding protein 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7254861
• Synonyms: ASPP2^flE4
• Gene: Trp53bp2 Location: Chr1:182236737-182289997 bp, + strand Genetic Position: Chr1, 84.93 cM
• Alliance: Trp53bp2^{tm1c(EUCOMM)Hmgu} page
• IMPC: Trp53bp2 gene page

Mutation origin

• Mutant Cell Line: HEPD0698_2_D09
• Germline Transmission: Earliest citation of germline transmission: J:321506
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 182262479 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 182263272. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:321506)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53bp2 Mutation: 52 strains or lines available

References

• Original: J:321506 Royer C, et al., ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis. Nat Commun. 2022 Feb 17;13(1):941
• All: 1 reference(s)