Trpm7^tm1.2Mfre
Targeted Allele Detail

Summary

• Symbol: Trpm7^tm1.2Mfre
• Name: transient receptor potential cation channel, subfamily M, member 7; targeted mutation 1.2, Marc Freichel
• MGI ID: MGI:6890462
• Gene: Trpm7 Location: Chr2:126633485-126718150 bp, - strand Genetic Position: Chr2, 61.76 cM, cytoband F2
• Alliance: Trpm7^tm1.2Mfre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:321349
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct replaced exon 2 with a tTA and a floxed neomycin resistance cassette driven by a tetracycline response element and CMV promoter. A third loxP site was inserted between exon 2 and 3. Cre-mediated recombination removed the selection cassette, tetracyline response element, and a portion of exon 2. (J:321349)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trpm7 Mutation: 101 strains or lines available

References

• Original: J:321349 Schutz A, et al., Trophectoderm cell failure leads to peri-implantation lethality in Trpm7-deficient mouse embryos. Cell Rep. 2021 Oct 19;37(3):109851
• All: 1 reference(s)