Tbce^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Tbce^{tm1c(EUCOMM)Hmgu}
• Name: tubulin-specific chaperone E; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6888292
• Synonyms: Tbce^fl
• Gene: Tbce Location: Chr13:14172534-14214223 bp, - strand Genetic Position: Chr13, 5.29 cM
• Alliance: Tbce^{tm1c(EUCOMM)Hmgu} page
• IMPC: Tbce gene page

Mutation origin

• Mutant Cell Line: HEPD0569_6_C04
• Germline Transmission: Earliest citation of germline transmission: J:321773
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 14189055 of Chromosome 13 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 14189856. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neo cassette and generates a "conditional ready" (floxed) allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:321773)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbce Mutation: 148 strains or lines available

References

• Original: J:321773 Efentakis P, et al., Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress. Eur Heart J. 2022 Feb 10;43(6):488-500
• All: 1 reference(s)