Cldn5^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cldn5^{tm1c(EUCOMM)Wtsi}
• Name: claudin 5; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6888275
• Gene: Cldn5 Location: Chr16:18595597-18597012 bp, + strand Genetic Position: Chr16, 11.63 cM
• Alliance: Cldn5^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cldn5 gene page

Mutation origin

• Mutant Cell Line: EPD0925_3_A11
• Germline Transmission: Earliest citation of germline transmission: J:295984
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo
• Mutation details: The Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo cassette was inserted at position 18595794 of Chromosome 16 (Build GRCm39) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 18596452. The critical artificial exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors. (J:295984)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cldn5 Mutation: 24 strains or lines available

References

• Original: J:295984 Frye M, et al., EphrinB2-EphB4 signalling provides Rho-mediated homeostatic control of lymphatic endothelial cell junction integrity. Elife. 2020 Sep 8;9:e57732
• All: 2 reference(s)