Rb1^tm1.1Ezac
Targeted Allele Detail

Summary

• Symbol: Rb1^tm1.1Ezac
• Name: RB transcriptional corepressor 1; targeted mutation 1.1, Eldad Zacksenhaus
• MGI ID: MGI:6887862
• Synonyms: Rb^deltaK4, Rb^K4
• Gene: Rb1 Location: Chr14:73430298-73563446 bp, - strand Genetic Position: Chr14, 38.73 cM, cytoband D3
• Alliance: Rb1^tm1.1Ezac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:321600
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Serine to alanine substitutions at amino acids 800 and 804 (S800A, S804A) and threonine to alanine substitutions at amino acids 814 and 819 (T814A, T819A) were introduced in exon 23 and a loxP flanked PGK-neo was inserted downstream of exon 23. These substitutions abolish phosphorylation at these sites. Cre-mediated recombination removed the neomycin cassette. (J:321600)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rb1 Mutation: 107 strains or lines available

References

• Original: J:321600 Jiang Z, et al., Hypophosphorylated pRb knock-in mice exhibit hallmarks of aging and vitamin C-preventable diabetes. EMBO J. 2022 Feb 15;41(4):e106825
• All: 1 reference(s)