Gt(ROSA)26Sor^{tm1.1(Cas9)Gidg}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(Cas9)Gidg}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Giusy Della Gatta
• MGI ID: MGI:6887630
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(Cas9)Gidg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:317226
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6NTac x 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Endonuclease, Reporter)
• Mutation: Insertion
• Mutation details: A BAC containing the Gt(ROSA)26Sor gene (BAC_ESr2-445b1_sfi_1) was modified by replacing part of intron 1 with a loxP site flanked neomycin resistance gene cassette, the Cas9 gene, sequence for P2A self-cleaving peptide sequence and the GFP reporter gene. The BAC replaced the endogenous locus through homologous recombination. The neo cassette was removed through subsequent cre-mediated recombination, leaving an allele expressing Cas9 and GFP driven by the Gt(ROSA)26Sor promoter. (J:317226)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

Notes

C57BL/6NTac x 129S6/SvEvTac ESCs

References

• Original: J:317226 Montasser ME, et al., Genetic and functional evidence links a missense variant in B4GALT1 to lower LDL and fibrinogen. Science. 2021 Dec 3;374(6572):1221-1227
• All: 1 reference(s)