Skic2^{tm2c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Skic2^{tm2c(EUCOMM)Wtsi}
• Name: SKI2 subunit of superkiller complex; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6885793
• Synonyms: Skiv2l^fl
• Gene: Skic2 Location: Chr17:35058202-35069180 bp, - strand Genetic Position: Chr17, 18.39 cM
• Alliance: Skic2^{tm2c(EUCOMM)Wtsi} page
• IMPC: Skic2 gene page

Mutation origin

• Mutant Cell Line: EPD0795_4_B02
• Germline Transmission: Earliest citation of germline transmission: J:321268
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 35065372 of Chromosome 17 upstream of the critical exons 3 to 10 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 35067878. The critical exons 3 to 10 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exons 3 to 10. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:321268)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Skic2 Mutation: 56 strains or lines available

References

• Original: J:321268 Yang K, et al., Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease. J Clin Invest. 2022 Jan 18;132(2):e146176
• All: 1 reference(s)