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Bscl2tm1d(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Bscl2tm1d(EUCOMM)Hmgu
Name: BSCL2 lipid droplet biogenesis associated, seipin; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:6883605
Synonyms: Bscl2 SKO
Gene: Bscl2  Location: Chr19:8814831-8826047 bp, + strand  Genetic Position: Chr19, 5.76 cM
Alliance: Bscl2tm1d(EUCOMM)Hmgu page
IMPC: Bscl2 gene page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:320952
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 8821809 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 8824055. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:320952)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Bscl2 Mutation:  33 strains or lines available
References
Original:  J:320952 Roumane A, et al., Bscl2 Deficiency Does Not Directly Impair the Innate Immune Response in a Murine Model of Generalized Lipodystrophy. J Clin Med. 2021 Jan 23;10(3)
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory