Zfp683^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zfp683^{tm1c(KOMP)Wtsi}
• Name: zinc finger protein 683; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6879484
• Synonyms: Hobit^fl
• Gene: Zfp683 Location: Chr4:133781149-133786307 bp, + strand Genetic Position: Chr4, 66.5 cM
• Alliance: Zfp683^{tm1c(KOMP)Wtsi} page
• IMPC: Zfp683 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:320757
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 133782391 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 133783708. The critical exon 3 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene generating a "conditional ready" (floxed) allele in which exon 3 is flanked by loxP sites. (J:320757)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp683 Mutation: 25 strains or lines available

References

• Original: J:320757 Friedrich C, et al., Effector differentiation downstream of lineage commitment in ILC1s is driven by Hobit across tissues. Nat Immunol. 2021 Oct;22(10):1256-1267
• All: 1 reference(s)