Gt(ROSA)26Sor^em14Mvw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^em14Mvw
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 14, Michael Wiles
• MGI ID: MGI:6877089
• Synonyms: DBA2.RosaBxb-GT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^em14Mvw page

Mutation origin

• Strain of Origin: DBA/2J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, RMCE-ready)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 mediated HDR using a single stranded oligo, which included homology arms, is used to insert a Bxb1 attP-GT site (48 bp) into intron 1 of the ROSA26 locus. (J:322700)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:322700 Low BE, et al., Efficient targeted transgenesis of large donor DNA into multiple mouse genetic backgrounds using bacteriophage Bxb1 integrase. Sci Rep. 2022 Mar 31;12(1):5424
• All: 1 reference(s)