Npnt^{em1(flpo)Ngai}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Npnt^{em1(flpo)Ngai}
• Name: nephronectin; endonuclease-mediated mutation 1, John Ngai
• MGI ID: MGI:6865658
• Gene: Npnt Location: Chr3:132587506-132656052 bp, - strand Genetic Position: Chr3, 61.52 cM
• Alliance: Npnt^{em1(flpo)Ngai} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:325783
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Npnt^{em1(flpo)Ngai} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Npnt (MGI:2148811)

• Mutation details: Using CRISPR/Cas9 technology, the P2A-FlpO sequence (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice) was inserted after the STOP codon of the nephronectin gene (Npnt) on chromosome 3 in v6.5 embryonic stem (ES) cells. Chimeric mice were generated by aggregating ES cells with morula-stage embryos. (J:325783)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Npnt (mouse) Summary of all recombinase alleles driven by Npnt. Alliance mouse Npnt gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Npnt Mutation: 43 strains or lines available

References

• Original: J:325783 A multimodal cell census and atlas of the mammalian primary motor cortex. Nature. 2021 Oct;598(7879):86-102
• All: 1 reference(s)