Gt(ROSA)26Sor^{tm3(CAG-Irs2)Mschu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-Irs2)Mschu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Markus Schubert
• MGI ID: MGI:6861841
• Synonyms: IRS2^tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-Irs2)Mschu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:318815
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The CAG-a loxP site flanked neo-Westphal stop-FLAG-mouse Irs2 cassette followed by an FRT site-framed IRESeGFP expression cassette were inserted into ROSA26 locus via homologous recombination in the ES cells. In presence of cre recombinase, a loxP site flanked neo-Westphal stop sequence is removed to allow expression of FLG epitope-tagged mouse Irs2 driven by CAG promoter. Expression of flp recombinase removes FRT site-framed IRESeGFP. (J:318815)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:318815 Zemva J, et al., Neuronal overexpression of insulin receptor substrate 2 leads to increased fat mass, insulin resistance, and glucose intolerance during aging. Age (Dordr). 2013 Oct;35(5):1881-97
• All: 1 reference(s)