Bcl2l11^em1Brg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Bcl2l11^em1Brg
• Name: BCL2 like 11; endonuclease-mediated mutation 1, David E Bergstrom
• MGI ID: MGI:6860661
• Synonyms: BCL2L11^h
• Gene: Bcl2l11 Location: Chr2:127967958-128004467 bp, + strand Genetic Position: Chr2, 62.23 cM, cytoband F3-G1
• Alliance: Bcl2l11^em1Brg page

Mutation origin

• Strain of Origin: C57BL/6NJ

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Using CRISPR/Cas9 technology, a 27,282-bp central segment of the human BCL2L11 gene flanked by 12,773- and 26,632-bp homology arms (consisting of the proximal and distal regions of the mouse Bcl2l11 gene), respectively including a conditionally removable loxP-flaked-segment (2.9-kbp) of intron 2, a cryptic human exon immediately 3 of this, and a native human exon some 20 kbp downstream replaced the mouse Bcl2l11 locus. (J:318662)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bcl2l11 Mutation: 37 strains or lines available

References

• Original: J:318662 Leidy-Davis T, et al., Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep. 2018 Oct 9;8(1):15028
• All: 1 reference(s)