H3f3b^em1Sraf
Endonuclease-mediated Allele Detail

Summary

• Symbol: H3f3b^em1Sraf
• Name: H3.3 histone B; endonuclease-mediated mutation 1, Shahin Rafii
• MGI ID: MGI:6860610
• Synonyms: H3.3B^-
• Gene: H3f3b Location: Chr11:115912738-115918788 bp, - strand Genetic Position: Chr11, 80.91 cM, cytoband E2
• Alliance: H3f3b^em1Sraf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:318597
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129S/SvEv x C57BL/6J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: ESC lines were generated through knock-in of a fluorescence (EYFP/mCherry) reporter and a stop codon to replace the three exons of the H3f3b gene via zinc finger nucleases. (J:318597)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H3f3b Mutation: 15 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:318597 Guo P, et al., Histone variant H3.3 maintains adult haematopoietic stem cell homeostasis by enforcing chromatin adaptability. Nat Cell Biol. 2022 Jan;24(1):99-111
• All: 1 reference(s)