H19^em2Keij
Endonuclease-mediated Allele Detail

Summary

• Symbol: H19^em2Keij
• Name: H19, imprinted maternally expressed transcript; endonuclease-mediated mutation 2, Keiji Tanimoto
• MGI ID: MGI:6856810
• Synonyms: inverted-ICR
• Gene: H19 Location: Chr7:142129267-142131883 bp, - strand Genetic Position: Chr7, 87.97 cM
• Alliance: H19^em2Keij page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Inversion
• Mutation details: Inversion of the H19 ICR sequence was induced at the mouse endogenous locus by CRISPR/Cas9 genome editing. Three lines carried correctly inverted ICR alleles; two of those three carried the artificial enzyme site. This is a mutant mouse line 23 established from these two F0 mice (lines 21 and 23). (J:317705)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H19 Mutation: 13 strains or lines available

References

• Original: J:317705 Matsuzaki H, et al., Orientation of mouse H19 ICR affects imprinted H19 gene expression through promoter methylation-dependent and -independent mechanisms. Commun Biol. 2021 Dec 17;4(1):1410
• All: 1 reference(s)