Prkag3^tm1.2Xen
Targeted Allele Detail

Summary

• Symbol: Prkag3^tm1.2Xen
• Name: protein kinase, AMP-activated, gamma 3 non-catalytic subunit; targeted mutation 1.2, Taconic Biosciences
• MGI ID: MGI:6856520
• Synonyms: AMPKgamma3^-, gamma3^-
• Gene: Prkag3 Location: Chr1:74778081-74788380 bp, - strand Genetic Position: Chr1, 38.54 cM
• Alliance: Prkag3^tm1.2Xen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:317473
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 5 to 10 were flanked by loxP sites. An FRT-flanked puromycin selection cassette was inserted downstream of exon 10 before the second loxP site. Flp-mediated recombination removed the selection cassette. Exons 5-10 were deleted via cre-mediated recombination in the germline. Deleting these exons deletes the cystathionine beta-synthase (CBS) 2 domain and parts of the CBS1 and 3 domains and is predicted to generate a frame shift from exon 4 to exon 11 and premature stop codon in exon 12. (J:317473)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkag3 Mutation: 26 strains or lines available

References

• Original: J:317473 Rhein P, et al., Compound- and fiber type-selective requirement of AMPKgamma3 for insulin-independent glucose uptake in skeletal muscle. Mol Metab. 2021 Mar 30;:101228
• All: 1 reference(s)