Tubb3^tm1.2Ece
Targeted Allele Detail

Summary

• Symbol: Tubb3^tm1.2Ece
• Name: tubulin, beta 3 class III; targeted mutation 1.2, Elizabeth C Engle
• MGI ID: MGI:6852608
• Gene: Tubb3 Location: Chr8:124138292-124148754 bp, + strand Genetic Position: Chr8, 72.1 cM
• Alliance: Tubb3^tm1.2Ece page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:270401
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 4 was replaced with a loxP site and a modified exon 4 that contains nucleotide substitutions resulting in the amino acid substitution of cysteine for arginine at position 262 (R262C), mimicking a mutation found in some congenital fibrosis of the extraocular muscles type 3 (CFEOM3) patients. An frt and loxP flanked neo cassette downstream of the modified exon 4 was removed by flp mediated recombination leaving the modified exon 4 floxed. Cre mediated recombination removed exon 4. (J:158992, J:270401)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tubb3 Mutation: 32 strains or lines available

References

• Original: J:270401 Latremoliere A, et al., Neuronal-Specific TUBB3 Is Not Required for Normal Neuronal Function but Is Essential for Timely Axon Regeneration. Cell Rep. 2018 Aug 14;24(7):1865-1879.e9
• All: 3 reference(s)