Cx3cr1^em1Mbur
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cx3cr1^em1Mbur
• Name: C-X3-C motif chemokine receptor 1; endonuclease-mediated mutation 1, Marie Burns
• MGI ID: MGI:6840220
• Synonyms: Cx3cr1^D2, Cx3cr1-Dendra2
• Gene: Cx3cr1 Location: Chr9:119877749-119897362 bp, - strand Genetic Position: Chr9, 71.37 cM
• Alliance: Cx3cr1^em1Mbur page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/cas9 genome editing is used to target two sites in exon 2. Double stranded oligonucleotide donor DNAs are created to replace the first 130 amino acids of the endogenous Cx3cr1 locus with Dendra2 (a green to red irreversibly photoswitchable monomeric fluorescent protein derived from octocoral Dendronephthya sp.), an SV40 nuclear localization signal, and a polyadenylation sequence. (J:312838)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cx3cr1 Mutation: 47 strains or lines available

References

• Original: J:312838 Miller EB, et al., Tracking distinct microglia subpopulations with photoconvertible Dendra2 in vivo. J Neuroinflammation. 2021 Oct 15;18(1):235
• All: 1 reference(s)