Pex10^em1Lutzy
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pex10^em1Lutzy
• Name: peroxisomal biogenesis factor 10; endonuclease-mediated mutation 1, Cathy Lutz
• MGI ID: MGI:6836733
• Synonyms: Pex10^{C286C,G287G,H288D}
• Gene: Pex10 Location: Chr4:155151487-155156863 bp, + strand Genetic Position: Chr4, 86.17 cM
• Alliance: Pex10^em1Lutzy page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/cas9 genome editing is used to generate the H288D missense mutation. (CAT to GAT). The H288D missense mutation that corresponds to the human H310D clinical mutation associated with Zellweger syndrome and peroxisome biogenesis disorders type 6A and 6B .Additionally, the following silent nucleotide changes were included to improve efficiency by preventing guide reassociation and retargeting of the modified allele with CRISPR/Cas9 (C286C [TGT to TGC] and G287G [GGC to GGT]). (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pex10 Mutation: 15 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)