Col1a1^{tm7(tetO-Cdkn2a)Jvd}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm7(tetO-Cdkn2a)Jvd}
• Name: collagen, type I, alpha 1; targeted mutation 7, Jan MA van Deursen
• MGI ID: MGI:6835763
• Synonyms: iL-p21
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm7(tetO-Cdkn2a)Jvd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:313419
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted into the 3 UTR a tetracycline response element (tetO), floxed stop cassette, myc- and flag-tagged mouse Cdkn1a coding sequence, woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), and poly-A signal. (J:313419)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:313419 Sturmlechner I, et al., p21 produces a bioactive secretome that places stressed cells under immunosurveillance. Science. 2021 Oct 29;374(6567):eabb3420
• All: 1 reference(s)