Gt(ROSA)26Sor^{tm6(CAG-CHRM3*,-ZsGreen)Jcbr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm6(CAG-CHRM3*,-ZsGreen)Jcbr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 6, Jens C Bruning
• MGI ID: MGI:6828737
• Synonyms: ROSA26lSlrSrhM3Dq
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm6(CAG-CHRM3*,-ZsGreen)Jcbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314806
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct consisting of a CAG promoter, a loxP-flanked neo stop cassette, a rox-flanked stop cassette, and DREADD (Designer Receptors Exclusively Activated by Designer Drugs) receptor hM3Dq, followed by 2A-NLS-ZsGreen and pA signal all inserted into the Gt(ROSA)26Sor locus was used to generate the mouse line by homologous recombination in ES cells. CAG promoter drives expression of hM3Dq and ZsGreen only after Cre and Dre mediated excision of the floxed and roxed stopper elements. (J:314806)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:314806 Biglari N, et al., Functionally distinct POMC-expressing neuron subpopulations in hypothalamus revealed by intersectional targeting. Nat Neurosci. 2021 Jul;24(7):913-929
• All: 2 reference(s)