Cdk6^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cdk6^{tm1c(EUCOMM)Wtsi}
• Name: cyclin dependent kinase 6; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6814677
• Synonyms: Cdk6^fl
• Gene: Cdk6 Location: Chr5:3391485-3581008 bp, + strand Genetic Position: Chr5, 2.04 cM, cytoband A2-A3
• Alliance: Cdk6^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cdk6 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314121
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 3440086 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 3441039. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:314121)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cdk6 Mutation: 38 strains or lines available

References

• Original: J:314121 Maurer B, et al., Inducible deletion of CDK4 and CDK6 - deciphering CDK4/6 inhibitor effects in the hematopoietic system. Haematologica. 2021 Oct 1;106(10):2624-2632
• All: 3 reference(s)