Kif13b^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kif13b^{tm1c(EUCOMM)Wtsi}
• Name: kinesin family member 13B; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:6810183
• Synonyms: Kif13b^tm1c
• Gene: Kif13b Location: Chr14:64889633-65047067 bp, + strand Genetic Position: Chr14, 33.77 cM, cytoband C3
• Alliance: Kif13b^{tm1c(EUCOMM)Wtsi} page
• IMPC: Kif13b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:312925
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 64951082 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 64953193. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:312925)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kif13b Mutation: 109 strains or lines available

References

• Original: J:312925 Waters SB, et al., KIF13B-mediated VEGFR2 trafficking is essential for vascular leakage and metastasis in vivo. Life Sci Alliance. 2022 Jan;5(1)
• All: 1 reference(s)