Gpbar1^tm1Ciphe
Targeted Allele Detail

Summary

• Symbol: Gpbar1^tm1Ciphe
• Name: G protein-coupled bile acid receptor 1; targeted mutation 1, Centre d'ImmunoPhenomique
• MGI ID: MGI:6810182
• Synonyms: TGR5-Tdt
• Gene: Gpbar1 Location: Chr1:74317709-74318783 bp, + strand Genetic Position: Chr1, 38.53 cM
• Alliance: Gpbar1^tm1Ciphe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:313904
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: A Hemagglutin (HA) Tag was inserted 3 in frame with the unique TGR5 exon after stop codon removal in order to generate a tagged TGR5 protein, and a tandem Tomato (Tdt) Tag was inserted downstream an IRES sequence in order to use Tdt expression as a surrogate marker for TGR5 expression. Further details were not specified. (J:313904)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpbar1 Mutation: 28 strains or lines available

References

• Original: J:313904 Castellanos-Jankiewicz A, et al., Hypothalamic bile acid-TGR5 signaling protects from obesity. Cell Metab. 2021 Jul 6;33(7):1483-1492.e10
• All: 1 reference(s)