Ptpn22^tm2.1Ciphe
Targeted Allele Detail

Summary

• Symbol: Ptpn22^tm2.1Ciphe
• Name: protein tyrosine phosphatase, non-receptor type 22 (lymphoid); targeted mutation 2.1, Centre d'ImmunoPhenomique
• MGI ID: MGI:6810180
• Synonyms: PTPN22^OST, Ptpn22^tm2Ciphe
• Gene: Ptpn22 Location: Chr3:103767111-103819563 bp, + strand Genetic Position: Chr3, 45.52 cM, cytoband F3
• Alliance: Ptpn22^tm2.1Ciphe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282407
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag)
• Mutation: Insertion
• Mutation details: Sequence for a loxP site, a OneSTrEPtag (OST) and a Gly-Ser-Gly spacer was inserted in-frame at the 5 end of the gene coding sequence and a second loxP site in inton 1. The FRT site flanked neomycin resistance gene cassette that was also inserted was removed through subsequent Flp-mediated recombination. The fusion of the OST to the protein can be used in affinity purification. (J:282407)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptpn22 Mutation: 51 strains or lines available

References

• Original: J:282407 Voisinne G, et al., Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics. Nat Immunol. 2019 Nov;20(11):1530-1541
• All: 2 reference(s)