Fyb1^tm1.1Ciphe
Targeted Allele Detail

Summary

• Symbol: Fyb1^tm1.1Ciphe
• Name: FYN binding protein 1; targeted mutation 1.1, Centre d'ImmunoPhenomique
• MGI ID: MGI:6810171
• Synonyms: FYB^OST
• Gene: Fyb1 Location: Chr15:6552334-6692794 bp, + strand Genetic Position: Chr15, 3.19 cM, cytoband A1
• Alliance: Fyb1^tm1.1Ciphe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282407
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: Sequence for a OneSTrEPtag (OST), two STOP codons, an IRES2, Kozak sequence, the cyan (teal) fluorescent reporter gene mTFP1, and an FRT site flanked neomycin resistance gene cassette, was inserted in-frame at the 3 end of the coding sequence. The neo cassette was removed through subsequent Flp-mediated recombination. (J:282407)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fyb1 Mutation: 41 strains or lines available

References

• Original: J:282407 Voisinne G, et al., Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics. Nat Immunol. 2019 Nov;20(11):1530-1541
• All: 1 reference(s)