Cd3e^tm1.1Arte
Targeted Allele Detail

Summary

• Symbol: Cd3e^tm1.1Arte
• Name: CD3 antigen, epsilon polypeptide; targeted mutation 1.1, TaconicArtemis
• MGI ID: MGI:6791371
• Synonyms: HuCD3epsilon
• Gene: Cd3e Location: Chr9:44910038-44920925 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Cd3e^tm1.1Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305166
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5 was replaced with a modified version of the equivalent, but 11 codons longer, exon from the human gene (exon 6). This exon codes for the binding epitope for the OKT3 anti-human CD3E antibody. The modification to the human sequence is the change of an arginine codon at the 3' end of the human exon to the mouse lysine codon. An FRT site flanked puromycin resistance gene cassette that was inserted into an intron, was removed through subsequent flp-mediated recombination. (J:305166)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd3e Mutation: 51 strains or lines available

References

• Original: J:305166 Crespo J, et al., A humanized CD3epsilon-knock-in mouse model for pre-clinical testing of anti-human CD3 therapy. PLoS One. 2021;16(2):e0245917
• All: 1 reference(s)