Itgam^tm1.1Dacb
Targeted Allele Detail

Summary

• Symbol: Itgam^tm1.1Dacb
• Name: integrin alpha M; targeted mutation 1.1, Daniel C Bullard
• MGI ID: MGI:6791321
• Synonyms: 77His
• Gene: Itgam Location: Chr7:127661812-127717663 bp, + strand Genetic Position: Chr7, 69.93 cM, cytoband F4
• Alliance: Itgam^tm1.1Dacb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:281653
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Single point mutation
• Mutation details: A mutation was generated in exon 3 changing proline codon 77 (CCC) to histidine (CAC)(p.P77H). A loxP site was inserted into intron 2 and a second loxP site and an FRT site flanked neomycin resistance gene cassette into intron 3. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with a floxed and mutated exon 3. The mutation mimics human SNP rs1143679 (p.R77H), which affects ligand binding of the encoded peptide. (J:281653)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itgam Mutation: 49 strains or lines available

References

• Original: J:281653 Avery JT, et al., Mice expressing the variant rs1143679 allele of ITGAM (CD11b) show impaired DC-mediated T cell proliferation. Mamm Genome. 2019 Oct;30(9-10):245-259
• All: 1 reference(s)